FastQCFastQC Report
Tue 28 Feb 2017
G120-M9_CGTACG-_BC7MKJANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M9_CGTACG-_BC7MKJANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10819095
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG518060.4788385719877679Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG61750.042.04058544
GCGTCGT83700.041.6053411
CGTCGTG84150.041.46108612
GATCGGA84400.041.425851
AGCGTCG84000.041.40418210
TGGTCGC62400.040.9669142
GTGGTCG64100.040.7727641
ATCGGAA85800.040.5707662
GATCTCG68000.040.44127334
GTCGTGT86450.040.3572713
TCGTGTA86250.040.27231614
CGTGTAG86700.040.240915
GAGCGTC87050.039.9029469
ATCTCGG70300.039.7440535
GGTCGCC64950.039.69722743
TCGGTGG71400.039.37824638
TCGGAAG88750.039.1472973
AGAGCGT89000.039.0781028
TCTCGGT70000.039.03437836
CGGTGGT69650.038.8186539
Produced by FastQC (version 0.11.2)