FastQCFastQC Report
Tue 28 Feb 2017
G120_M6M7M8.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120_M6M7M8.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37521597
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT591440.1576265530489014TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA184500.040.773681
CTCGTAT167400.039.9714344
TCTCGTA156650.039.33316843
ATCGGAA190650.039.309532
CGTCTGA193700.038.92009716
ACACGTC198000.038.09459713
CGTTTCG29850.037.81501433
ACGTCTG199950.037.6790615
ATCTCGT165100.037.2679742
GTCACGT190850.037.0433529
TCGGAAG202750.036.9359633
AGTCACG192650.036.92574328
AACGATC73700.036.7005638
CGGAAGA207150.036.064244
CACGTCT211350.035.85491614
CGATCTC77250.035.099440
GATCTCG79850.034.01160441
TATCTCG71650.033.9755541
ACGATCT80250.033.89688539
CACACGT226150.033.5431612
Produced by FastQC (version 0.11.2)