FastQCFastQC Report
Tue 28 Feb 2017
G120_M9M10M11.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120_M9M10M11.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37315970
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT576130.15439234193831755TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA263450.041.2039761
CCGTACG77450.041.02380433
CTCGTAT227000.040.86599744
CGTACGT78100.040.7377434
TCTCGTA212600.040.52361343
CGTCTGA268400.040.2789116
ATCGGAA270050.040.153642
ACGTCTG274600.039.446715
ACACGTC276250.039.17116513
ATCTCGT222350.038.60986742
TCGGAAG282300.038.416953
ACCGTAC82850.038.3765132
CGTAATC72700.038.17249338
GTACGTA84050.037.7948135
TATCTCG152900.037.78141441
CGGAAGA285450.037.755464
CACGTCT290650.037.3667314
CACCGTA87450.036.53765531
TACGTAA86600.036.13027236
GCACACG300800.035.9837411
Produced by FastQC (version 0.11.2)