FastQCFastQC Report
Tue 28 Feb 2017
G120_M9M10M11.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120_M9M10M11.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37315970
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1627090.4360304716720482Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG188050.042.01133744
GATCGGA264700.041.0134851
GCGTCGT263700.040.8858311
TGGTCGC191150.040.86881642
CGTCGTG264150.040.84105312
AGCGTCG264850.040.70802710
GATCTCG209400.040.37519534
ATCGGAA269400.040.2333222
GTGGTCG198200.040.1920941
GGTCGCC197750.039.87192543
CGTGTAG272850.039.6750215
GTCGTGT274050.039.4531313
TCGTGTA274600.039.2859414
GAGCGTC275000.039.1815349
ATCTCGG219450.039.06751335
TCGGTGG221700.038.92892538
TCGGAAG279650.038.687373
TCTCGGT218050.038.5616436
CGGTGGT216150.038.3806539
AGAGCGT282150.038.2354058
Produced by FastQC (version 0.11.2)