FastQCFastQC Report
Tue 28 Feb 2017
G121-M11_GGCTAC-_BC7MKJANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M11_GGCTAC-_BC7MKJANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25036570
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG787610.31458382677818886Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT128900.041.5759111
AGCGTCG129650.041.16357810
CGTCGTG130850.041.04038612
GATCGGA131950.040.869311
ATCGGAA133350.040.3240932
TCGTGTA134900.039.90714614
GTCGTGT137250.039.33582313
CGTGTAG138450.039.0110115
GAGCGTC136850.038.8691149
GATCTCG113000.038.51011334
TGGTCGC102700.037.91542442
AGAGCGT141500.037.8390248
TCGGAAG143350.037.6184733
GTGGTCG107550.037.43142741
AAGAGCG142800.037.3097727
GTCGCCG106300.036.90040644
ATCTCGG119500.036.8934235
CGGAAGA149400.035.6385774
TCGGTGG125150.035.56062738
TCTCGGT121400.035.44602636
Produced by FastQC (version 0.11.2)