FastQCFastQC Report
Tue 28 Feb 2017
G121-M12_CTTGTA-_BC7MKJANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M12_CTTGTA-_BC7MKJANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25397701
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC3054051.2024907293774347TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA381300.042.716171
ATCGGAA382800.042.428782
CGTATGC382000.042.0578644
CGTCTGA392400.041.95399516
CTCGTAT372000.041.92755542
TCGTATG384150.041.8859343
AATCTCG349200.041.8320739
TCTCGTA352800.041.76606841
ACACGTC394500.041.67472513
ACGTCTG399750.041.2375715
ATCTCGT356550.041.21594240
TCGGAAG396800.041.0658153
CACGTCT403650.040.78454614
CGGAAGA402100.040.5391434
CACACGT414550.039.73879212
GCACACG416800.039.5336611
GTAATCT377500.038.45026437
TAATCTC394100.037.0047538
TGTAATC392700.036.867636
CCTTGTA428550.036.73983433
Produced by FastQC (version 0.11.2)