FastQCFastQC Report
Tue 28 Feb 2017
G121-M2_CGATGT-_BC7MKJANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M2_CGATGT-_BC7MKJANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30110099
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC13177784.376531608215569TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1602950.043.53511
ATCGGAA1603400.043.3409542
TCGGAAG1621150.042.965763
ACACGTC1633800.042.8762813
CGGAAGA1625050.042.867134
CGTCTGA1627950.042.8451916
ACGTCTG1636500.042.75982715
CACGTCT1644850.042.62428714
CGTATGC1608550.042.57617644
CGATGTA1564300.042.49242434
CTCGTAT1579050.042.4522942
CACCGAT1587600.042.45146631
ACCGATG1583100.042.43591732
TATCTCG1476200.042.42598739
TCGTATG1614600.042.42572843
CCGATGT1574400.042.40159633
TCTCGTA1491250.042.3934841
GTCACCG1618800.042.2645429
CACACGT1660550.042.24255812
TCACCGA1610000.042.23990230