FastQCFastQC Report
Tue 28 Feb 2017
G121-M4_TGACCA-_BC7MKJANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M4_TGACCA-_BC7MKJANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26788213
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC8260243.0835352847164534TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1010800.043.3942071
ATCGGAA1014050.043.1411132
CGTCTGA1028900.042.84417316
ACACGTC1033750.042.7727613
CGTATGC1015550.042.7712544
TCGGAAG1027650.042.670643
ACGTCTG1036050.042.65892815
TCGTATG1020700.042.57291843
CTCGTAT980950.042.56257242
AATCTCG920150.042.4723839
TCTCGTA930150.042.45919441
CGGAAGA1031100.042.4532364
CACGTCT1045050.042.35447314
ATCTCGT930950.042.3047640
GCACACG1053650.041.95747811
CACACGT1056150.041.90316412
GACCAAT964350.041.06852335
ACCAATC941350.040.92952736
CCAATCT954900.040.4166937
GAACTCC1082250.040.38866821
Produced by FastQC (version 0.11.2)