FastQCFastQC Report
Tue 28 Feb 2017
G121-M4_TGACCA-_BC7MKJANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M4_TGACCA-_BC7MKJANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26788213
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6551442.4456427907303855Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT985250.043.44346611
AGCGTCG984800.043.3924510
CGTCGTG987850.043.3804312
GTCGCCG748500.043.31986644
GATCGGA989100.043.304781
ATCGGAA990000.043.2493672
TCGTGTA994800.043.05391714
TGGTCGC747750.043.0308642
GTCGTGT999450.043.02718413
GAGCGTC993850.042.9595269
CGTGTAG998450.042.9185615
GATCTCG829850.042.9079534
GTGGTCG773450.042.89993741
AGAGCGT1002000.042.7752848
ATCTCGG845100.042.75032435
TCGGAAG1003850.042.7379763
GGTCGCC759100.042.73233843
AAGAGCG999950.042.6763847
TCTCGGT834350.042.56011636
CGGAAGA1002450.042.492594