FastQCFastQC Report
Tue 28 Feb 2017
G121-M6_GCCAAT-_BC7MKJANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M6_GCCAAT-_BC7MKJANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26031400
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6959272.673413646596034Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1047350.043.436581
CGTCGTG1048500.043.40323612
AGCGTCG1044100.043.40141310
GCGTCGT1046200.043.3893111
ATCGGAA1048200.043.3001022
GTCGCCG792550.043.2743944
GTCGTGT1058250.043.15585313
TCGTGTA1053600.043.11896514
CGTGTAG1056100.043.03564515
GAGCGTC1051750.043.016719
TGGTCGC791550.042.9482242
TCGGAAG1059600.042.9192163
AGAGCGT1061200.042.8478368
GATCTCG872850.042.83331734
GTGGTCG820900.042.76479341
GGTCGCC801900.042.75876643
AAGAGCG1057700.042.7362177
ATCTCGG890200.042.62342535
CGGAAGA1060450.042.550784
TCTCGGT875400.042.5446236