FastQCFastQC Report
Tue 28 Feb 2017
G121-M7_CAGATC-_BC7MKJANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M7_CAGATC-_BC7MKJANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24795774
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1420510.572883911589128Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT223250.042.23554211
CGTCGTG224650.042.1194812
AGCGTCG223200.042.0950310
GATCGGA225950.041.9601171
ATCGGAA227450.041.7310872
TCGTGTA230400.041.09779414
CGTGTAG230250.041.05768615
GTCGTGT231550.041.0455413
TGGTCGC173500.040.62593542
GAGCGTC231650.040.5878379
GATCTCG194050.040.53094534
GTGGTCG180850.040.1777441
AGAGCGT234950.040.1657078
GTCGCCG178850.039.90269544
ATCTCGG199700.039.80253235
TCGGAAG238850.039.7299463
AAGAGCG236050.039.6900447
TCGGTGG205700.039.0573238
CGGAAGA240550.039.010134
GGTCGCC183800.038.92381343
Produced by FastQC (version 0.11.2)