FastQCFastQC Report
Tue 28 Feb 2017
G121-M8_ACTTGA-_BC7MKJANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M8_ACTTGA-_BC7MKJANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26750286
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1181460.44166256764507117Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG184900.041.8691912
GATCGGA186400.041.749851
GCGTCGT185100.041.74067711
AGCGTCG185400.041.56509810
ATCGGAA189550.041.0092282
TCGTGTA190000.040.6773814
GTCGTGT192000.040.55112513
CGTGTAG192950.040.08967215
GTGGTCG150450.039.37706841
TGGTCGC146650.039.3185242
GAGCGTC196050.039.2957959
GATCTCG161700.039.22449534
AGAGCGT198750.038.9153988
GTCGCCG151350.038.56281344
ATCTCGG167750.038.4652235
TCGGAAG203750.038.269873
AAGAGCG201500.038.1551067
TCGGTGG175200.037.61946538
TCTCGGT169450.037.5338236
GGTCGCC155750.037.44500443
Produced by FastQC (version 0.11.2)