FastQCFastQC Report
Tue 28 Feb 2017
G121-M9_GATCAG-_BC7MKJANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M9_GATCAG-_BC7MKJANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26719790
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1446190.5412430262363589TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG190850.041.55544743
CTCGTAT185100.041.44791842
GATCGGA192650.041.3580441
CGTATGC192200.041.2396844
TCTCGTA173900.040.9941241
ACGATCA191000.040.99112332
ATCGGAA194150.040.832142
CGATCAG192900.040.58296233
GTCACGA195700.040.42504529
TCACGAT195650.040.3330930
CACGATC195200.040.26696431
ATCTCGT178350.039.94662540
CGTCTGA202350.039.71105616
ACACGTC203850.039.5485213
GATCTCG179750.039.4774239
ACGTCTG208100.038.77232715
AGTCACG205100.038.72296528
TCGGAAG209250.037.9604263
CACGTCT213700.037.84911314
CGGAAGA215650.036.786994
Produced by FastQC (version 0.11.2)