FastQCFastQC Report
Tue 28 Feb 2017
G121-M9_GATCAG-_BC7MKJANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M9_GATCAG-_BC7MKJANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26719790
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1144000.4281470775032289Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG187650.041.61949512
GCGTCGT187800.041.52768311
AGCGTCG188000.041.40011610
GATCGGA188450.041.108961
ATCGGAA189800.040.850882
TCGTGTA191900.040.5498614
GTCGTGT195500.039.94922613
CGTGTAG195200.039.875615
GATCTCG163700.039.2560334
GAGCGTC199650.039.1275759
TGGTCGC149100.038.92326442
ATCTCGG168350.038.7073135
AGAGCGT202750.038.6794178
GTGGTCG156050.038.35870441
TCGGAAG206100.037.833513
AAGAGCG206400.037.8145077
CGGAAGA209350.037.046494
GTCGCCG160500.036.76179544
TCTCGGT173800.036.5437636
TCGGTGG181700.036.1283338
Produced by FastQC (version 0.11.2)