FastQCFastQC Report
Tue 28 Feb 2017
G121_M10M11M12.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121_M10M11M12.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77847605
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1896920.2436709517267744TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA759550.042.2269741
ATCGGAA764700.041.7890172
CTCGTAT739250.041.47336242
TCGTATG761250.041.40344643
CGTATGC762650.041.36412444
TCTCGTA705400.041.14120541
CGTCTGA787350.040.9515616
ACACGTC792050.040.75030513
ACGTCTG801200.040.4138915
ATCTCGT719350.040.27625340
TCGGAAG808200.039.705683
CACGTCT825150.039.21428714
CGGAAGA818850.039.1431774
AATCTCG374350.038.7690839
CACACGT845950.038.21373412
GCACACG852300.037.9458511
TATCTCG253000.037.21735839
ACGGCTA150250.035.3979932
GAACTCC959550.033.4692121
CGGCTAC159650.033.07627533
Produced by FastQC (version 0.11.2)