FastQCFastQC Report
Tue 28 Feb 2017
G121_M10M11M12.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121_M10M11M12.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77847605
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4694250.6030050635469133Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT732550.042.47072211
CGTCGTG735250.042.44946312
AGCGTCG735150.042.26820410
GATCGGA742200.042.1361121
ATCGGAA748100.041.765242
TCGTGTA751250.041.4673814
GTCGTGT758000.041.2893813
CGTGTAG761600.040.9905115
GAGCGTC761400.040.790699
TGGTCGC572500.040.61733642
GATCTCG635850.040.4987934
AGAGCGT773900.040.3205648
GTGGTCG596700.040.2631441
ATCTCGG652950.039.9094235
TCGGAAG785900.039.879513
GTCGCCG590850.039.81023844
AAGAGCG783800.039.6826637
CGGAAGA797700.039.00554
GGTCGCC604300.038.88038643
TCTCGGT658250.038.87939536
Produced by FastQC (version 0.11.2)