Basic Statistics
Measure | Value |
---|---|
Filename | G121_M1M2M3.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 82778347 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC | 97926 | 0.11829905228718811 | TruSeq Adapter, Index 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 238340 | 0.0 | 43.19468 | 1 |
ATCGGAA | 239170 | 0.0 | 42.891865 | 2 |
CGTATGC | 239055 | 0.0 | 42.512474 | 44 |
ACACGTC | 243930 | 0.0 | 42.485733 | 13 |
CGTCTGA | 243475 | 0.0 | 42.476643 | 16 |
CTCGTAT | 234360 | 0.0 | 42.41189 | 42 |
TCGTATG | 239775 | 0.0 | 42.39845 | 43 |
TCTCGTA | 220770 | 0.0 | 42.38322 | 41 |
TCGGAAG | 243215 | 0.0 | 42.299423 | 3 |
ACGTCTG | 245085 | 0.0 | 42.29354 | 15 |
CGATGTA | 158225 | 0.0 | 42.13208 | 34 |
CGGAAGA | 244455 | 0.0 | 42.080727 | 4 |
ATCTCGT | 222585 | 0.0 | 41.990253 | 40 |
CACGTCT | 247405 | 0.0 | 41.92901 | 14 |
CACCGAT | 161290 | 0.0 | 41.880474 | 31 |
TATCTCG | 150365 | 0.0 | 41.80864 | 39 |
ACCGATG | 161175 | 0.0 | 41.761543 | 32 |
CCGATGT | 160520 | 0.0 | 41.687714 | 33 |
CACACGT | 251245 | 0.0 | 41.32578 | 12 |
TCACCGA | 164800 | 0.0 | 41.31178 | 30 |