G121_M1M2M3.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G121_M1M2M3.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82778347
Sequences flagged as poor quality	0
Sequence length	50
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	1511816	1.8263423404673689	Illumina Single End PCR Primer 1 (100% over 50bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCGTCGT	231450	43.289177	11
CGTCGTG	232170	43.27627	12
AGCGTCG	230860	43.256413	10
GTCGCCG	176035	43.1105	44
GATCGGA	232840	43.106304	1
ATCGGAA	233210	42.955185	2
TCGTGTA	234030	42.866978	14
GTCGTGT	235195	42.833782	13
TGGTCGC	175065	42.74233	42
GAGCGTC	233560	42.73347	9
CGTGTAG	235190	42.69952	15
GATCTCG	195340	42.62392	34
GTGGTCG	180780	42.541214	41
GGTCGCC	178455	42.480198	43
AGAGCGT	236045	42.44668	8
ATCTCGG	198870	42.40889	35
TCGGAAG	237185	42.328754	3
AAGAGCG	236065	42.257896	7
TCGGTGG	202295	42.0822	38
TCTCGGT	195930	42.06935	36