FastQCFastQC Report
Tue 28 Feb 2017
G121_M7M8M9.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121_M7M8M9.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78265850
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3745970.4786212632968274Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT587800.041.8249211
CGTCGTG589750.041.8135612
AGCGTCG589700.041.6136610
GATCGGA591950.041.5996631
ATCGGAA597650.041.165442
TCGTGTA606600.040.66394414
GTCGTGT612000.040.4954613
CGTGTAG609200.040.49400715
GATCTCG513000.039.8146834
GAGCGTC617250.039.759739
TGGTCGC464500.039.52313242
AGAGCGT625350.039.3659258
GTGGTCG480650.039.28331841
ATCTCGG530550.038.86623835
TCGGAAG637800.038.6427883
AAGAGCG636450.038.465567
GTCGCCG485600.038.3857144
CGGAAGA646050.037.8394174
TCTCGGT537350.037.67834536
GGTCGCC497200.037.46357343
Produced by FastQC (version 0.11.2)