FastQCFastQC Report
Mon 23 Nov 2015
G122-M11_ATGTCA-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M11_ATGTCA-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17072930
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220840.12935096670577342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA21200.018.67664138
GTCGGAT16600.018.5731181
ACGTTCG26400.016.094436
GTGCGAT24700.016.03020139
TCGGATT20800.015.8755432
TGCGATC29050.013.78149340
CGTTCGA30850.013.2019737
TACGGTA20700.013.1762531
GGGGCGT30400.013.16863734
GCGTGCG31500.012.98824737
GTTACGA10500.012.3704364
TGGGTCG34150.012.36542615
ACGGTAC22650.012.23609532
GGGCGTG34000.012.03340535
TTACGGT21950.012.0250630
TTACGAC10650.011.7827745
GTCGAAA7150.011.704221
GTTCGAA35800.011.6834188
TTGACTA23100.011.61865442
GGGTCGT36200.011.604382516
Produced by FastQC (version 0.11.2)