FastQCFastQC Report
Mon 23 Nov 2015
G122-M11_ATGTCA-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M11_ATGTCA-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17072930
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG34700.015.3430693
CACCCGG38700.013.6998524
GCACGGT57300.013.59141932
CCGGACA42400.013.17904812
CGCGGTA12450.013.07691517
TGGCACG63200.012.7059930
ACACGGA46000.012.10059816
GGCACGG66300.011.87957631
TCTAGTC67950.011.7852723
CACGGAC45950.011.77856817
ACGGACA49000.011.09031118
CGGACAC51900.011.021481513
CCCGGAC51700.010.97817211
CGGTGAA72450.010.74913235
TCTAACG14750.010.7387285
CGGTAAT15400.010.71478519
ACGGTGA74250.010.63681534
CTAGTCT76300.010.495378524
GCTTGAC77850.010.48478316
CACGGTG77000.010.39978333
Produced by FastQC (version 0.11.2)