FastQCFastQC Report
Mon 23 Nov 2015
G122-M12_CCGTCC-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M12_CCGTCC-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7144544
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG6200.013.8491016
CGTGCGA5650.013.62623838
TCGGATT7200.013.1482022
GTCGATT4153.6379788E-1211.1430751
TTAGTCG7950.010.8001028
CGTTCGA7150.010.7773177
TACGACG3351.3831595E-810.5130699
GTCGGTA5050.010.4653491
CGAACGT5500.010.39702814
TAGTCGG8400.010.2197369
GTGCGAT7850.010.08763139
CGCGTTT1750.002798629710.0666691
TCGGTTT10800.09.9885572
GTCCGTT7750.09.9448951
CGGTTTG6550.09.7477043
GTCGGTT9900.09.5646081
TACGAGG6700.09.5295386
GTCGACA4754.2200554E-109.2719321
AATCCGA6650.09.26038324
ACGTGCG5803.6379788E-129.1007217
Produced by FastQC (version 0.11.2)