FastQCFastQC Report
Mon 23 Nov 2015
G122-M12_CCGTCC-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M12_CCGTCC-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7144544
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19450.033.1454731
TATGCCG15450.029.0484663
ATGCCGT16900.023.3012244
GTATGCC29100.020.4138892
CCGTCTT13350.019.4448227
TGCCGTC19750.018.9363925
GCCGTCT17600.018.2491846
CGACAAT5800.017.8297841
TACGCAC5300.015.358664527
TCGACCG8700.014.66755120
GTTATCG4850.014.51572913
ACACGGA21150.014.14752416
TTATCGT5150.014.09783814
TCACCCG18800.013.9254943
CCGGACA19750.013.92362312
CGTCTTC17450.013.8675758
ACGGACA19300.013.67967618
CTCGATA2653.4924597E-1013.28465751
CTAAAAC24500.013.111821
CGAACGC8250.013.0682771
Produced by FastQC (version 0.11.2)