FastQCFastQC Report
Mon 23 Nov 2015
G122-M13_GTCCGC-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M13_GTCCGC-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18308790
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG22700.030.61911832
GATCGGA27050.026.4595011
ATCGGAA33000.021.6150022
ACACGTC33850.021.50762613
ACGTCTG33900.021.4099715
AGTCACG33050.021.22956828
CGTCTGA34750.021.0760916
GTCACGT33400.021.00756329
CGTCCGC33950.020.73202333
GTCCGCA36200.019.50445434
CTCGTAT37200.018.8654444
CGCACAT37900.017.35274537
CGGAAGA42700.017.1695774
TCACGTC43400.017.13004330
CACACGT44200.016.8715112
CACGTCT43700.016.75986514
TCGGAAG48450.015.0864663
GCACACG53550.014.46220411
CACGTCC51850.014.29593631
TCTCGTA47350.014.03145343
Produced by FastQC (version 0.11.2)