FastQCFastQC Report
Tue 16 Aug 2016
G122-M15_GTGGCC-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M15_GTGGCC-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20376418
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGTTACCTTGTTTTTTTTTGGTCTTTTTGTTATTTTGTCTTTTTT242130.11882853993277916No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241880.11870584908495693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA35850.028.196111
ACGTCTG39450.025.64576115
ACACGTC42800.023.69180713
CGTCTGA43200.023.57224516
ACGTGGC45500.022.62442632
CTCGTAT43050.021.77016344
AGTCACG47100.021.71512828
GTCACGT46800.021.5259729
ATCGGAA49800.020.3341792
TATCTCG45400.019.76851741
CGGAAGA53350.019.7656944
CACGTCT52200.019.38218514
CGTGGCC52800.019.24656733
CACACGT54600.019.13761512
GCCTTAT49600.018.84753837
TCACGTG57500.017.97931330
TCGGAAG60150.017.604413
TCTCGTA56750.016.74719443
GTGCCAG108450.016.4889181
GCACACG67000.016.0574411
Produced by FastQC (version 0.11.2)