FastQCFastQC Report
Mon 23 Nov 2015
G122-M16_GTTTCG-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M16_GTTTCG-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18823871
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG18950.025.30428133
GATCGGA24100.020.286591
ACGTTTC28750.016.83183732
ACACGTC29600.016.71924813
ACGTCTG30150.016.7049115
AGTCACG31200.016.56686628
CTCGTAT29400.015.6396744
GTCACGT32600.015.3837729
CGTCTGA31900.015.30583816
ATCGGAA33700.014.4368192
AGTTACC48600.013.9975166
TCGGAAT33900.013.56079737
GTTTCGG37400.013.17416534
CGGAAGA40500.013.1549484
TTCGGAA38350.012.90496936
GTTACCT53500.012.8801037
TTTCGGA38650.012.7481635
CACGTCT41300.012.46148614
AATCTCG35550.012.43750241
CGGAATC34650.012.25188938
Produced by FastQC (version 0.11.2)