FastQCFastQC Report
Mon 23 Nov 2015
G122-M18_GAGTGG-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M18_GAGTGG-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19554346
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT21150.022.1551211
CGTCGTG23300.019.8279212
TCGTGTA26850.017.45318214
GATCGGA27500.017.0423531
CGTGTAG27450.016.83124715
GTAGGGA28650.016.58723618
GATCTCG24250.016.14841334
GTCGTGT30950.015.42503613
AGCGTCG33400.015.14900310
AGAGCGT33150.014.865238
GTAGATC28200.014.66670531
GAGCGTC35250.014.6660659
TGTAGGG33850.014.10411917
ATCTCGG27600.013.94895435
TCTCGGT29450.013.59550936
ATCGGAA35550.012.9968442
GTGTAGG38950.012.65279816
GTCGCCG32100.011.58178444
GGTCGCC35000.011.06212143
TAGGGAA45050.010.69533119
Produced by FastQC (version 0.11.2)