FastQCFastQC Report
Mon 23 Nov 2015
G122-M19_ACTGAT-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M19_ACTGAT-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19378029
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC317140.163659575491398No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG198400.10238399374879663Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT38050.033.70612711
CGTCGTG39150.032.8164612
GTCGTGT43150.029.9798613
TCGTGTA44150.029.2518914
GATCTCG38150.028.60282934
GATCGGA45600.028.5177441
CGTGTAG45200.028.42633815
AGCGTCG46750.027.6214110
GAGCGTC48600.026.7963689
AGAGCGT49350.026.70138
GTAGGGA49100.026.6166718
GTCGCCG37750.026.10667444
TCTCGGT42150.025.73066536
GTAGATC44300.025.52663631
ATCGGAA51100.025.1888542
ATCTCGG43850.024.83372135
TGTAGGG53800.024.37321317
GTGTAGG55850.023.7937316
GGTCGCC42400.023.76232743
GTGGTCG44400.022.39490941
Produced by FastQC (version 0.11.2)