FastQCFastQC Report
Wed 2 Dec 2015
G122-M1_ACAGTG-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M1_ACAGTG-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17911180
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1475640.8238653176395971TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA179200.042.0432471
TCGTATG184300.040.53733443
CGTATGC185500.040.21602244
ACACGTC188150.040.07372313
ACGTCTG190450.039.76115
CTCGTAT180100.039.5633542
CGTCTGA191150.039.46581616
ATCGGAA190800.039.413692
CACGTCT196950.038.48290314
CGGAAGA197450.038.1104744
CACACGT198650.038.01611312
TCTCGTA179750.037.05600441
AGTGATC181050.036.62911636
GATCTCG181450.036.5859639
TCGGAAG206800.036.5152443
ATCTCGT189300.035.1865640
GCACACG214750.035.1705311
GAACTCC225550.033.5539121
AGAGCAC232550.032.5081868
GTCACAC233150.032.32135429
Produced by FastQC (version 0.11.2)