FastQCFastQC Report
Wed 2 Dec 2015
G122-M1_ACAGTG-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M1_ACAGTG-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17911180
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1131570.6317674212419282Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA179150.040.4646514
GATCGGA181900.040.2695271
CGTCGTG181250.040.07832712
GCGTCGT182050.040.02204511
CGTGTAG182050.039.8563215
GTCGTGT183950.039.53988613
GTAGGGA184700.039.36851518
AGAGCGT186150.039.2941788
ATCGGAA186500.039.121162
GTAGATC161100.038.8243331
GATCTCG153950.038.75489434
TGTAGGG190700.038.32599317
ATCTCGG157950.038.19076535
GAGCGTC192650.037.8311589
TCTCGGT156550.037.70308736
GTGTAGG194100.037.69998616
AGCGTCG193650.037.65841310
AAGAGCG194650.037.4088557
GAGTGTA187950.036.7431627
AGTGTAG185600.036.2628
Produced by FastQC (version 0.11.2)