FastQCFastQC Report
Mon 23 Nov 2015
G122-M20_ATTCCT-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M20_ATTCCT-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17398335
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA16600.019.2373391
ATCGGAA21600.014.8803022
ACACGTC24950.013.57592713
GTCGGTT15450.013.2568251
CTCGTAT23650.013.11617344
CGTCTGA25700.012.83674716
TATCTCG22500.012.514194541
ACGTCTG27200.012.20980215
AGTTACC47050.011.4640776
GTTACCT51800.011.2203617
CTCCGTT22700.010.3810911
TCTCGTA29900.010.22734243
CACACGT33900.010.18762512
GTCGGTA9750.010.1646621
GTCGTTT34800.010.0624311
CTCCGAT20350.09.9565411
CTCGGTT26600.09.9353851
CGCGATA2007.564885E-49.9105471
CGCCTAT4754.1836756E-119.7366771
TCGGTTT24250.09.6229172
Produced by FastQC (version 0.11.2)