FastQCFastQC Report
Wed 2 Dec 2015
G122-M2_GCCAAT-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M2_GCCAAT-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16624533
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2348281.4125389266573685TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA286450.042.6569061
ATCGGAA291650.041.7639772
CGTATGC289350.041.36199644
TCGTATG290100.041.27010343
TATCTCG268250.041.18977739
ACACGTC295300.041.14623313
CGCCAAT290800.041.12539333
ACGCCAA291800.041.0749132
ACGTCTG295900.041.00669515
CGTCTGA295500.040.995116
GTCACGC293650.040.95096629
CTCGTAT289450.040.73781242
AGTCACG296700.040.56548328
CACACGT300100.040.52987712
CGGAAGA304250.040.093614
CACGTCT303500.040.04602414
TCACGCC302850.039.72874530
TCTCGTA280750.039.5528141
TCGGAAG310800.039.3410843
CACGCCA306050.039.1696131
Produced by FastQC (version 0.11.2)