FastQCFastQC Report
Wed 2 Dec 2015
G122-M3_CAGATC-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M3_CAGATC-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17112523
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG713050.41668315069613054Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT117500.039.78570611
TCGTGTA118200.039.6277814
GATCGGA120400.039.1082341
CGTCGTG119500.039.04688312
GTCGTGT124000.037.8977613
AGCGTCG123450.037.72523510
CGTGTAG124400.037.68813315
GATCTCG103900.036.73712534
GTAGATC109000.036.4108931
GTAGGGA128900.036.3389118
AGAGCGT129450.036.316778
GAGCGTC128750.036.291889
ATCGGAA129200.036.272622
TCTCGGT106000.035.67619736
GTGTAGG133850.035.37309316
TGTAGGG134250.035.2021517
ATCTCGG110600.035.08777235
GTCGCCG99800.034.87149444
AAGAGCG137400.034.151337
GGTCGCC101550.034.11881643
Produced by FastQC (version 0.11.2)