FastQCFastQC Report
Mon 23 Nov 2015
G122-M7_GGCTAC-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M7_GGCTAC-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16258404
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC230120.14153910802068886TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA33700.033.74477432
GATCGGA34500.033.6360661
TCGTATG37300.030.9661543
ATCGGAA38100.030.563942
CGTATGC39250.029.03533444
ACACGTC40100.028.74242813
CTCGTAT39400.028.47720342
CGGCTAC40450.028.22245233
AGTCACG43100.027.14998228
CGTCTGA42250.027.01741616
ACGTCTG44400.026.10550715
TCACGGC46150.025.07032430
CACACGT48250.024.07257712
GTCACGG48350.023.8840929
CACGTCT50000.023.1821914
CGGAAGA51650.023.0579994
TCTCGTA47500.022.41549941
CACGGCT53300.021.66596431
GCACACG53250.021.5675611
ATCTCGT50600.021.04202540
Produced by FastQC (version 0.11.2)