FastQCFastQC Report
Mon 23 Nov 2015
G122-M7_GGCTAC-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M7_GGCTAC-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16258404
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT29550.036.32996411
CGTCGTG34200.031.519512
AGCGTCG36000.029.94297210
GATCGGA37050.029.8119491
CGTGTAG36150.029.76043515
TCGTGTA36950.029.11609814
GTCGTGT38650.027.60706913
GATCTCG31300.026.2169334
GAGCGTC41650.026.0923569
ATCTCGG34650.024.18995735
TCTCGGT33950.023.58705136
GTAGGGA47150.023.3309518
AGAGCGT48350.023.2047928
TGTAGGG49900.022.44198817
GTAGATC38000.022.28961431
GTGTAGG49900.022.26562716
GTCGCCG33000.022.2652344
ATCGGAA49600.022.0460512
AAGAGCG51300.021.5702237
GTGGTCG36150.020.81225441
Produced by FastQC (version 0.11.2)