FastQCFastQC Report
Mon 23 Nov 2015
G122-M8_CTTGTA-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M8_CTTGTA-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21036503
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG240030.11410166414066063Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT44650.036.6561611
CGTCGTG50000.032.8671912
CGTGTAG51050.032.02161415
TCGTGTA52650.031.42459114
AGCGTCG53650.030.9576910
GTCGTGT54450.030.3846813
GATCGGA54950.029.4714571
GAGCGTC59100.028.1401679
GATCTCG47800.027.89072434
AGAGCGT65550.025.6062688
TCTCGGT52450.025.29143536
ATCTCGG53950.025.03702435
GTAGGGA65750.025.0300218
GTAGATC58300.024.34012231
AAGAGCG68750.024.1264867
TGTAGGG68200.024.06632617
ATCGGAA67200.023.9342582
GTCGCCG54450.023.63420744
GTGGTCG55750.023.32006841
GTGTAGG71950.023.20952816
Produced by FastQC (version 0.11.2)