FastQCFastQC Report
Mon 23 Nov 2015
G122-M9_AGTCAA-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M9_AGTCAA-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17195852
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT17400.014.03521921
TCGACCG18300.012.98447720
TTGGCGC21250.011.4910326
GTCGACC21900.011.25189619
TGTCGAC22050.011.07557218
CGCTTCG24650.010.3521610
CTGTCGA24500.010.23742117
TTTGGCG25350.09.7197815
CCGTGCG25850.09.6170524
GGCGCTT27550.09.1028418
GACGCGA9100.08.94544622
GCTTCGC28900.08.82986111
AACGCGT7300.08.1364438
TTCGCTG32800.07.914819213
CGATCGT10400.07.82640088
TGCGCTT33850.07.73434727
ACCGTGC34950.07.67974423
TAATCGA11250.07.6265134
AATCGAA17800.07.292035
ACGCGAT7854.0017767E-117.28681623
Produced by FastQC (version 0.11.2)