FastQCFastQC Report
Wed 16 Mar 2016
G126-G127-M1M20-NEBNext22_CGTACG-_AC8KUAANXX_L005_001.R1_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext22_CGTACG-_AC8KUAANXX_L005_001.R1_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8696775
Sequences flagged as poor quality0
Sequence length20-50
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC102335511.767063077980055No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2714203.1209270102997952No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCC2427082.7907816403206933No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1921532.2094742016437126No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC1496401.7206378226411516No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCC1110261.2766341546147852No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCC872521.0032684529610114No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC614310.7063652905818536No Hit
CTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC602400.6926705589140802No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC455900.5242173104397895No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGC349950.4023905413213518No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGC349860.4022870546840639No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT311230.3578682902570206No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC296580.3410229654095915No Hit
CGGGTGGGGCCCAAGTCCTTCTGATCGAGGCC275070.316289659097769No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC265550.30534307257575366No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT244560.2812076890571505No Hit
CGCGGGGGGCCCAAGTCCTTCTGATCGAGGCC236230.2716294258503871No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC227000.261016296270744No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCC212160.2439524996334848No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCC211630.24334307832501126No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA201010.23113165512503195No Hit
CGTCGGGGGCCCAAGTCCTTCTGATCGAGGCC186580.21453929761319568No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT171990.19776296385729192No Hit
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCC171430.19711904700305571No Hit
CTCGCCCGTCACGTTGAACGCACGTTCG169010.194336406311535No Hit
CGTGGGGGCCCAAGTCCTTCTGATCGAGGCC166060.19094434431154078No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCC151890.17465094819631416No Hit
CTCGCCCGTCACGTTGAACGCACGTTC151070.1737080699454683No Hit
TGGGGGGGGCCCAAGTCCTTCTGATCGAGGCC144810.16650999939632793No Hit
CCTGGGGGGCCCAAGTCCTTCTGATCGAGGCC140360.161393160108201No Hit
CATGGGGGGCCCAAGTCCTTCTGATCGAGGCC127390.14647958582348053No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGC126490.14544471945060095No Hit
CTAAACCATTCGTAGACGACCTGCTTC122090.1403853727387451No Hit
NTTGGGGGGCCCAAGTCCTTCTGATCGAGGCC121660.13989093658281374No Hit
GGTGGGGGCCCAAGTCCTTCTGATCGAGGCC118980.13680933449468338No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCAC116140.1335437561624855No Hit
NGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC115170.1324284001828264No Hit
ATCACAAGAAAGACGTGGTCCTGACAGAC113200.13016319267774548No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGG109870.12633418709809097No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCA107100.12314909837267261No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCT106120.12202224387775928No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC97650.1122830014574368No Hit
GTCGGGGGCCCAAGTCCTTCTGATCGAGGCC97360.11194954451506449No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACG94840.1090519186710016No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT91620.10534939675914347No Hit
CTAAACCATTCGTAGACGACCTGCTT89810.10326816549813005No Hit
TCTGTGACGTATGATGGATTCAATACATT88720.10201482733542032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG6950.0295.1675444
TCACCCG4900.0287.217544
ATCACGC5550.0224.9379343
CGTAATA11850.0208.605937
GTAATAT7250.0207.6897438
TCACACG50250.0205.0702244
CACGCGT4250.0196.4418644
ATCACCC4900.0190.2332343
CACGTCT62500.0186.2493444
GCACCCG5400.0185.5284344
CACAGGT8250.0185.0465244
TCGTAAT12950.0184.8983336
CCCGGGT22500.0184.0933834
CGGCCCC38200.0178.6362530
GATCACG6650.0178.0594242
GCCATAT17000.0176.4056930
TAATATC8900.0173.5161639
GGCCCCG39600.0171.6192631
TAATAGC6500.0165.1005139
GCCCCGG42400.0162.1132232
Produced by FastQC (version 0.11.2)