FastQCFastQC Report
Wed 16 Mar 2016
G126-G127-M1M20-NEBNext25_ACTGAT-_AC8KUAANXX_L005_001.R1_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext25_ACTGAT-_AC8KUAANXX_L005_001.R1_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6840745
Sequences flagged as poor quality0
Sequence length20-50
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC5827128.518253494319698No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1569132.2937998712128578No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCC1343331.9637188639541454No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1012521.4801311845420346No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC975151.4255026316578092No Hit
CTCGCCCGTCACGTTGAACGCACGTTC918711.3429969981339753No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCC592040.8654612911313022No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCC502510.7345837332044974No Hit
CTAAACCATTCGTAGACGACCTGCTT399300.5837083534030285No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC397350.5808577866884381No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC351610.5139937243677406No Hit
CTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC341630.499404670105376No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC303040.4429926857381762No Hit
CTCGCCCGTCACGTTGAACGCACGTTCG299280.4374962083808123No Hit
CTCGCCCGTCACGTTGAACGCACGTT275650.40295318711631556No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCC267790.39146321051289007No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT261700.3825606713888619No Hit
CCGTGGCGCAATGAAGGTGAAGGGC228470.33398409091407444No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGC222530.32530082615270706No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGC207600.30347571792253625No Hit
CGTGGGGGCCCAAGTCCTTCTGATCGAGGCC201370.29436852272669134No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCC198030.2894860135847777No Hit
GTTGGGGGGCCCAAGTCCTTCTGATCGAGGCC186240.27225104867963945No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC161770.23648009098424222No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGC160330.2343750571026986No Hit
CTAAACCATTCGTAGACGACCTGCTTC151950.22212492937538236No Hit
CCTTGGCGCAATGAAGGTGAAGGGC136200.19910112129599922No Hit
CGCGGGGGGCCCAAGTCCTTCTGATCGAGGCC133290.19484719866037983No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT117400.17161873450917992No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCC113670.16616611202434822No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC110290.1612251297190584No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCC110210.16110818339230595No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCC109620.16024570423250684No Hit
GCTGAGAAGACGGTCGAACTTGACTATC97670.14277684667386373No Hit
GTTGGGGGGCCCAAGTCCTTCTGATCGAGGC97210.14210440529503732No Hit
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCC96910.14166585656971573No Hit
GCCGCCGGTGAAATACCACTACTCTCAT95670.13985318850505318No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCC85710.12529337082437658No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGT85120.12443089166457746No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGC83750.12242818581894223No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC82010.11988460321207704No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCC80640.1178818973664418No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGT78000.11402266858361186No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGG75740.11071893485285594No Hit
CTAGACCATTCGTAGACGACCTGCTT72900.10656734025314495No Hit
CATGGGGGGCCCAAGTCCTTCTGATCGAGGCC72300.10569024280250178No Hit
NTTGGGGGGCCCAAGTCCTTCTGATCGAGGCC70680.10332207968576522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG5150.0282.2359344
CGTAATA6900.0219.2455637
GTAATAT4600.0218.3732638
TCACCCG3600.0216.8309544
ATCACGC4400.0207.1791243
TTCGATT6650.0202.3830644
CACAGGT8550.0201.4835744
CGGCCCC13250.0192.961730
TCGTAAT8500.0184.3897636
GCCATAT10650.0178.8754930
CCCGGGT10150.0176.6727334
GATCACG4850.0176.6543342
TTGCGGC13300.0174.7669227
GGCCAGA53150.0172.5450629
TAATATC6150.0171.0824639
GCCAGAT51650.0170.585430
CACGTCT75000.0168.3206344
AGGCCAG55300.0167.8700428
TCACACG36800.0163.1108444
GGCCATA11100.0157.8370829
Produced by FastQC (version 0.11.2)