FastQCFastQC Report
Wed 16 Mar 2016
G126-G127-M1M20-NEBNext27_ATTCCT-_AC8KUAANXX_L005_001.R1_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext27_ATTCCT-_AC8KUAANXX_L005_001.R1_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7532186
Sequences flagged as poor quality0
Sequence length20-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2240942.975152233362267No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1373361.823321941332835No Hit
CTAAACCATTCGTAGACGACCTGCTT876811.1640843707258424No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCC789751.0485003955027135No Hit
CTCGCCCGTCACGTTGAACGCACGTTC764221.0146058528028916No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC658090.8737038623315994No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCC601930.7991438342069619No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC575560.7641340774112588No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC558010.7408340686223097No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCC525830.6981107476634273No Hit
CTCGCCCGTCACGTTGAACGCACGTT484680.6434785333235266No Hit
CTCGCCCGTCACGTTGAACGCACGTTCG434360.5766718984369212No Hit
CCGTGGCGCAATGAAGGTGAAGGGC420990.5589214074108101No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT409380.543507555442736No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT332990.44208945450895665No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGC321060.4262507590757849No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC316660.42040916143069224No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT299880.3981314322296343No Hit
CTAGACCATTCGTAGACGACCTGCTT272640.3619666322631969No Hit
CCTTGGCGCAATGAAGGTGAAGGGC252590.3353475339031723No Hit
CTAAACCATTCGTAGACGACCTGCT215110.28558774305361023No Hit
CTCGCCCGTCACGTTGAACGCACGT214630.2849504778559637No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC199460.2648102423386783No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCC198900.2640667662747574No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCC194570.25831810313765485No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCC191190.253830694037561No Hit
CTAAACCATTCGTAGACGACCTGCTTC185180.24585160270869572No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG182310.24204128788110116No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC165340.2195113078726415No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC154180.20469489202736096No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGC151040.20052611552609031No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCC150770.20016765385241414No Hit
CTAGACCATTCGTAGACGACCTGCT149770.198840018023984No Hit
CTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC134310.17831476811645386No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGC119500.15865248149740327No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC119460.15859937606426608No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGT113980.15132393172446884No Hit
GCTGAGAAGACGGTCGAACTTGACTATC112210.14897401630814747No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCAC106500.1413932157278113No Hit
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCAC91860.12195662719959384No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG91340.12126625656881015No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCC87140.11569018608940353No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGT86940.11542465892371749No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCC86440.11476084100950242No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT85220.11314112529881763No Hit
GGTGGGGGCCCAAGTCCTTCTGATCGAGGCC83820.11128243513901542No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCC81210.10781730562681273No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCC79880.1060515499750006No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC77680.10313075115245428No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC76970.10218812971426888No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC75680.10047547949559396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGC25000.0228.6163244
CCCGGGT95900.0143.1798234
CGGCCCC144800.0133.79530
CCCCGGG149000.0133.3704533
TACGTAG28750.0127.64622543
GGCCCCG156900.0125.73675531
GCCCCGG158950.0125.6807732
GTACGTA25350.0122.7166442
GCGGCCC152500.0114.62630529
TGCGGCC146550.0112.7248928
TTGCGGC146400.0111.0011127
CGTACGT29050.0110.5469741
CCGGGTA6150.0109.2058935
GGTTTCG24350.0108.9189936
GTTTCGT25100.0107.9113737
TTCGTAC27900.0107.8926839
CACGTCT107700.0106.688544
TCGTACG29000.0105.6502840
TTTCGTA27300.0104.07342538
GGGCGCT6900.0103.5444
Produced by FastQC (version 0.11.2)