FastQCFastQC Report
Mon 1 May 2017
G124_G125_M4-M9.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M4-M9.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92158302
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC296150.039.4850944
TCGTATG300550.038.93773743
CTCGTAT288000.038.87840742
TCTCGTA265500.038.58030741
GATCGGA307750.038.4567531
CGTCTGA315950.037.91344516
ATCGGAA321700.036.9763952
ACGTCTG328500.036.5922915
ACACGTC337700.035.64086513
ATCTCGT287300.035.61460540
TCGGAAG341450.034.9532133
CGGAAGA346850.034.2949334
GCACACG375750.032.02216711
CACACGT379800.031.77839312
GATCTCG117750.029.63785239
CACGTCT417000.029.01621414
CGATCAG67750.027.91288833
AGTCACG147200.027.89092628
CGCCAAT78650.027.6511533
AATCTCG133550.027.43227439
Produced by FastQC (version 0.11.2)