FastQCFastQC Report
Mon 1 May 2017
G124_G125_M4-M9.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M4-M9.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92158302
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN949970.10308024121364562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG268150.040.06206510
GTCGCCG164050.040.0106444
CGTCGTG267800.039.9791612
GCGTCGT268750.039.76767311
GATCGGA290800.037.545681
GTCGTGT288150.037.2735613
GAGCGTC287250.037.260099
CGTGTAG290100.036.9675415
TGGTCGC174050.036.75142742
GATCTCG203400.036.6745534
ATCGGAA298250.036.545462
TCGTGTA290550.036.47508214
GTGGTCG183300.036.43269341
GGTCGCC181000.035.88712743
AGAGCGT303800.035.4987378
AAGAGCG307650.035.089557
TCGGAAG312700.035.014763
TCGGTGG230250.033.739438
TCTCGGT217700.033.7216136
CGGAAGA323400.033.324634
Produced by FastQC (version 0.11.2)