FastQCFastQC Report
Mon 1 May 2017
G124_G125_M7M8M9.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M7M8M9.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44825146
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC130900.039.50911744
TCGTATG133700.038.74847843
CTCGTAT127900.038.70042842
GATCGGA135800.038.0679051
TCTCGTA118700.037.97780641
CGTCTGA141200.037.62494716
ACGTCTG145950.036.49090215
ATCGGAA143350.036.1677552
ATCTCGT127200.035.37087240
ACACGTC150950.035.3548513
TCGGAAG148200.035.0433543
CGATCAG55500.034.19270333
CGGAAGA153250.033.7594534
GTCACGA58100.033.23009529
ACGATCA58850.032.6946932
CACGATC60700.031.80687131
GCACACG170500.031.21628611
CACACGT172650.030.79943812
GATCTCG56850.028.31443439
CACGTCT190500.028.06108314
Produced by FastQC (version 0.11.2)