FastQCFastQC Report
Mon 1 May 2017
G124_G125_M10-M15.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M10-M15.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111614428
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1340860.12013321431885132TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1667500.041.572916
ACGTCTG1683850.041.29208415
GATCGGA1686150.040.8210451
CGTATGC679100.040.63602444
ATCGGAA1727100.039.9022372
ACACGTC1749800.039.7834913
TCGTATG697350.039.52028343
TCGGAAG1744400.039.452063
CCGTATC352950.039.35155538
CGGAAGA1755500.039.041044
CACGTCT1817700.038.34584414
CACACGT1819750.038.32015612
GCACACG1817800.038.28656811
TCCGTAT372150.038.27858437
CGTATCT364500.038.25543639
AGTTCCG416400.038.05941834
GAACTCC1866500.037.08707421
CGACGCT104550.036.79450617
CACGACG105800.036.71340615
CGGCTAC189850.036.2999933
Produced by FastQC (version 0.11.2)