FastQCFastQC Report
Mon 1 May 2017
G124_G125_M10-M15.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M10-M15.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111614428
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7440660.6666396211787243Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1173110.10510379536237018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG927150.042.99946644
TGGTCGC915450.042.2248942
GGTCGCC949200.041.8127443
GATCTCG1052700.041.7445134
GTGGTCG967600.041.7358341
TCGGTGG1103400.041.28061338
TCTCGGT1048450.041.0366336
ATCTCGG1087850.040.99455635
CGGTGGT1031350.040.72672339
CTCGGTG1065050.040.51660537
GATCGGA1584950.040.23311
CGTGTAG1556400.040.16254415
ATCGGAA1590250.039.968912
CGTCGTG1576800.039.59629412
AGCGTCG1581650.039.539510
GCGTCGT1577800.039.52150311
TCGGAAG1618100.039.4433143
GTCGTGT1597400.039.06627713
TCGTGTA1581750.039.04848514
GAGCGTC1599600.038.9812139
Produced by FastQC (version 0.11.2)