FastQCFastQC Report
Mon 1 May 2017
G124_G125_M10M11M12.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M10M11M12.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53115842
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2902590.5464640850464161Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN541900.10202229308536614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG365000.042.85425644
TGGTCGC362200.041.88606642
GGTCGCC376050.041.3729343
GATCTCG425300.041.25977734
GTGGTCG386900.041.09376541
TCGGTGG444900.040.72189338
TCTCGGT424100.040.47074536
GATCGGA637100.040.4112051
ATCTCGG441100.040.34591735
CGTGTAG624650.040.22349515
ATCGGAA639850.040.114712
CGTCGTG628000.040.03326412
CGGTGGT416450.039.94416839
AGCGTCG631500.039.9406710
GCGTCGT629200.039.89778511
CTCGGTG432550.039.82905637
TCGGAAG655850.039.3426133
TCGTGTA630450.039.32302514
GAGCGTC640000.039.2825369
GTCGTGT639600.039.26538513
Produced by FastQC (version 0.11.2)