FastQCFastQC Report
Mon 1 May 2017
G124_G125_M13M14M15.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M13M14M15.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58498586
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4538070.7757572123196277Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN631210.10790175338597073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG555900.043.09168644
TGGTCGC548550.042.42202842
GGTCGCC565700.042.11975543
GTGGTCG579000.042.05603441
GATCTCG626200.042.01074634
TCGGTGG659600.041.6386838
TCTCGGT629300.041.25343736
CGGTGGT613150.041.19872739
ATCTCGG648150.041.17202435
CTCGGTG631600.041.02142737
GATCGGA938300.040.2757641
CGTGTAG922650.040.2745215
ATCGGAA944050.039.91372
TCGGAAG952750.039.6048853
CGTCGTG942350.039.4886112
GCGTCGT941950.039.4152211
AGCGTCG944850.039.38730210
GTCGTGT949600.039.12256213
TCGTGTA941050.039.09538314
GAGCGTC954250.038.9298029
Produced by FastQC (version 0.11.2)