FastQCFastQC Report
Mon 3 Apr 2017
G124-G125-M1M19-NEBNext20_GTGGCC-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext20_GTGGCC-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19837857
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN198960.10029309113378526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG8850.022.6331212
GCGTCGT9000.021.76912711
AGCGTCG10050.019.27574510
GTCGCCG7450.017.42008244
GTCGTGT14850.013.78361913
TCGTGTA15100.013.6989914
TGGTCGC9900.013.33137442
GAGCGTC16750.012.6167569
CGTGTAG16950.012.07246915
GTGGTCG13000.011.33673941
ATCGGAA18850.011.089742
GATCGGA19550.010.35392951
AGAGCGT20100.010.1854288
GATCTCG14700.010.02366334
GGTCGCC14550.09.52439843
AAGAGCG23500.09.1799357
ATCTCGG18000.08.91917735
TCGGTGG18700.08.46870238
TCGGAAG26700.08.1630933
TCTCGGT18150.07.997572436
Produced by FastQC (version 0.11.2)