FastQCFastQC Report
Thu 6 Apr 2017
G124_G125_M20M21.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M20M21.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35148509
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT960640.27330889057057867TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG116550.041.44079633
CCGTACG91600.041.1310633
CGTACGT92200.040.887634
CTCGTAT205800.040.30924644
GATCGGA225400.040.1695371
TCTCGTA196150.039.72492243
CGTCTGA228150.039.67062416
ATCGGAA229750.039.3496862
AATCTCG197250.039.32650441
ACCGTAC96250.039.2126732
ACACGTC231350.039.10264613
ACGTCTG232000.038.8604615
CGTAATC88300.038.5830838
GTACGTA98300.038.41706535
GTTTCGG126100.038.33754734
TCGGAAT117500.038.2604337
CGGAATC115300.038.22738338
ATCTCGT206450.037.82865542
TTTCGGA127650.037.76827235
TCGGAAG240100.037.635123
Produced by FastQC (version 0.11.2)