FastQCFastQC Report
Thu 6 Apr 2017
G124_G125_M20M21M22.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M20M21M22.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58810685
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT960650.16334616745239408TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA653650.042.0741351
CGATCAG433500.041.79619233
CGTATGC442100.041.69694544
CGTCTGA663700.041.6315916
ATCGGAA661400.041.534862
ACGATCA437350.041.4637832
GTCACGA440600.041.3866429
TCGTATG447500.041.2528643
ACACGTC670000.041.24320213
ACGTCTG669900.041.21013315
CACGATC439800.041.13770731
GATCTCG407300.040.71027839
TCACGAT447600.040.6802630
CGTTTCG118800.040.67407233
AGTCACG574050.040.5920428
TCGGAAG678000.040.5276573
CCGTACG93200.040.4717733
CGGAAGA681400.040.2029344
CGTACGT94150.040.06394634
CACGTCT694800.039.80930714
Produced by FastQC (version 0.11.2)